Part:BBa_K2996706:Design

mRFP downstream of activation unit containing target

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 52
  Illegal NheI site found at 75
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 39
  Illegal AgeI site found at 661
  Illegal AgeI site found at 773
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

None.

Source

None.

References

[1]. Gilbert L A, Larson M H, Morsut L, etc. CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes[J]. Cell, 2013, 154(2): 442-451. [2]. Mathew R, Chatterji D. The evolving story of the omega subunit of bacterial RNA polymerase[J]. Trends in microbiology, 2006, 14(10): 450-5. [3]. Gourse R L, Ross W, Gaal T. UPs and downs in bacterial transcription initiation: The role of the alpha subunit of RNA polymerase in promoter recognition[J]. Molecular Microbiology, 2000, 37(4): 687-695. [4]. Murakami K S, Masuda S, Darst S A. Structural Basis of Transcription Initiation: RNA Polymerase Holoenzyme at 4 Å Resolution[J]. Science, 2002, 296(5571): 1280-1284.